Globin-Reduction Controls and their Expected Results

Sample	Rx	Globin Reduction	Total RNA Input (µg)	Replicates	Expected Result
Jurkat RNA	1	No	2	3	Optimal GeneChip array results are expected. This reaction is used as a reference to determine success of the GeneChip Target Labeling.
Jurkat RNA	2	Yes	2	3	Optimal GeneChip array results are expected. This reaction is used to verify that the globin reduction procedure does not affect non-globin transcripts.
Jurkat RNA + Globin RNA	3	No	2	3	Sub-optimal GeneChip results are expected due to a loss in assay sensitivity. This reaction is used to demonstrate the effects of high amounts of globin mRNAs on array analysis.
Jurkat RNA + Globin RNA	4	Yes	2	3	Optimal GeneChip array results similar to those obtained using the Jurkat RNA control are expected. This reaction is used to demonstrate the increase in assay sensitivity due to the globin reduction procedure.