This kit provides both positive and negative control RNA samples to validate the globin-reduction procedure in conjunction with the GeneChip® Blood RNA Concentration Kit (P/N 900585).
The Globin-Reduction RNA Controls box contains 2 vials of RNA:
Reagent
Description
Jurkat RNA
Purified from Jurkat cells that contain negligible amounts of globin mRNAs, this control represents RNA purified from the leukocyte fraction of whole blood.
Jurkat +
Globin RNA
This control RNA is the Jurkat RNA described above but spiked with in vitro-synthesized, polyadenylated globin transcripts. The Jurkat + Globin RNA control mimics RNA purified from unfractionated whole blood.
The kit provides sufficient Jurkat RNA and Jurkat + Globin RNA for six reactions each, for a total of twelve reactions.
Recommended globin-reduction controls and their expected results. View Table
