VeriQuest Taq DNA Polymerase
Chemically modified, full length hot start Taq DNA polymerase for PCR
Advantages of USB VeriQuest Taq DNA Polymerase:
- Chemically modified Taq polymerase for hot start PCR, eliminating the formation of non-specific products and primer dimers
- Room temperature reaction set-up
- Exceptional performance, offering high specificity and sensitivity
This hot start Taq polymerase has no polymerase activity prior to the initial heat activation step, allowing reaction assembly at room temperature as well as higher specificity and sensitivity. The initial incubation step of 95°C for 10 min before PCR cycling removes the blocking chemical moiety resulting in activation of the polymerase.
USB VeriQuest Taq Polymerase is a chemically modified, full length Taq DNA polymerase for hot start PCR.
Free from detectable non-specific nucleases.
20mM Tris-HCl, pH 8.5, 100mM KCl, 1mM DTT, 0.1mM EDTA, 50% glycerol and stabilizers.
Unit Definition: One unit of enzyme is defined as the amount that catalyzes the incorporation of 10 nmol of total nucleotide into acid-insoluble form in 30 minutes at 74°C.
Assay Conditions: The reaction mixture contains 25mM TAPS, pH 9.3 (at 25°C), 50mM KCl, 2mM MgCl2, 1mM β-ME, 200µM each dATP, dGTP, dTTP, 100µM [α-32P]-dCTP (0.05 to 0.1 Ci/mmole), 250 µg/ml activated salmon sperm DNA and VeriQuest Taq DNA Polymerase. After incubation at 74°C for 10 minutes, acid insoluble material is determined (50 µl reaction volume).
Functional Assay: Functionally tested for PCR according to the standard Tested User Friendly PCR protocol. This protocol is based upon the amplification of a 500 bp lambda DNA.
Concentration: 5 units/ul
Functionally Tested VeriQuest Taq 10X PCR Reaction Buffer (included, PN 71188):
150mM Tris-HCl, pH 8.0, 500mM KCl, 15mM MgCl2.
Functionally Tested MgCl2 (included, PN 71167):
25mM MgCl2 solution
Shipped on dry ice. Store at -20°C.