Ligate-IT Rapid Ligation Kit
Can be used for a variety of ligation procedures such as conventional vector cloning, TA cloning, linker or adaptor ligation, and library construction
Description:
Ligate-IT™ Rapid Ligation Kit provides high-quality reagents for fast and easy ligation
reactions.
Quick and Efficient
Ligation reactions are complete following room temperature incubation in just 5
minutes for cohesive-ends and 10 minutes for blunt-ends (see Figs. 1-2). Transform competent
cells immediately or store reactions at -20°C for later transformation.
Figure 1: Rapid ligation of cohesive-end and blunt-end fragments.
Figure 2: Ligate-IT Rapid Ligation Kit transformation efficiency of cohesive-end and blunt-end ligations.
Convenient
Ligate-IT Rapid Ligation Kit includes USB T4 DNA Ligase, Nuclease-Free Water, and a specially
formulated 5X Reaction Buffer which enhances the activity of T4 DNA Ligase, especially on
blunt-end fragments. All of the components are in a ready-to-use format and do not require any
additional reagents, such as ATP.
Flexible
The kit can be used for a variety of ligation procedures such as conventional vector cloning,
TA cloning, linker or adaptor ligation, and library construction.
Kit
Components 25
Reaction 100 Reaction
Pack
Size Pack Size
Ligate-IT T4 DNA Ligase (PN
78401) 25
μl 100
μl
5X Ligate-IT Reaction Buffer (PN
78402) 100
μl
400 μl
Water, Nuclease-Free (PN
71786) 1
ml
2 x 1 ml
Recombinant T4 DNA Ligase is supplied in 25mM Tris-HCl (pH 7.6), 100mM NaCl, 1mM DTT, 0.5mM EDTA, 50% glycerol.
Storage
Kit shipped on dry ice. Store components at -20°C in a non-frost-free freezer.
Tested User Friendly™ Functional Test:
Cohesive- and blunt-end ligations of a 1200 bp insert into pUC19 yielded ≥1 x 106
transformants per microgram of linearized, dephosphorylated vector.
References:
1. SAMBROOK, J. AND RUSSELL, D. W. (2001) “Molecular Cloning: A Laboratory Manual,” Cold Spring
Harbor Laboratory Press, 1.84-1.125.
FAQs
MSDS / Material Safety Data Sheets
Ligate-IT Rapid Ligation Kit_MSDS
Product Spotlight
USB_Ligate-IT_Product Spotlight
Protocol, Brief
Ligate-IT Rapid Ligation Kit_Brief Protocol