Acrylamide
MB Grade
Molecular Formula:
CH2=CHCONH2
Molecular Weight:
71.08
PRODUCT SPECIFICATIONS
Form: White/clear crystalline powder
Assay: ≥ 99.9%
Identity: By IR
A (410 nm, 35%, 1 cm): ≤ 0.02
A (290 nm, 1%, 1 cm): ≤ 0.1
A (280 nm, 7mM, 1 cm): ≤ 0.04
A (252 nm, 7mM, 1 cm): ≤ 1.20
Insoluble Matter (H2O): ≤ 0.005%
Insoluble Matter (MeOH): ≤ 0.005%
pH (10%, 0.1N NaCl): 5.5 – 7.0
Melting Point: 84° ± 1°C
Acrylic Acid: ≤ 0.001%
Conductivity (35%): ≤ 5 μmhos/cm
Iron (Fe): ≤ 1 ppm
Lead (Pb): ≤ 1 ppm
Gel Performance: Passes test
Applications:
• Basic component for preparing polyacrylamide gels.
• Used at a 19:1 ratio with bis-acrylamide.
DNA Size Separation by Acrylamide Percentage:
DNA Size Range (bp) Acrylamide Conc.(%)
100 – 1,000 3.5
80 – 500 5.0
60 – 400 8.0
40 – 200 12.0
10 – 100 20.0
CAS:
79-06-1
