RecA Protein
Source:
E. coli strain containing an overproducing clone of E. coli RecA Protein.
Description:
E. coli RecA Protein, encoded by the recA gene of E. coli, participates in general recombination, repair of DNA and regulation of repair(1,2). It has been observed in vitro to have DNA-dependent ATPase activity, promote proteolytic cleavage of repressors(3) and to catalyze DNA strand pairing and exchange(4,5). In the presence of ATP, RecA Protein promotes the strand exchange of single-strand DNA fragments with homologous duplex DNA(6). This protein will form helical filaments with single-stranded and duplex DNA(7,8).
Properties:
Molecular Weight: 37.8 kDa(8)
ATP dependent
Purity:
Greater than 98% pure as determined by SDS-PAGE. Tested for contaminating non-specific endonucleases, exonucleases and ribonucleases.
Storage Buffer:
20mM Tris-HCl (pH 7.5), 1mM DTT, 0.1mM EDTA,50% glycerol.
Concentration:
5 mg/ml, measured by A280(3)
References:
- RADDING, C. M. (1978) Ann. Rev. Biochem. 47, 847-880.
- RADDING, C. M. (1982) Ann. Rev. Genet. 16, 405-437.
- CRAIG, N. L. AND ROBERTS, J. W. (1981) J. Biol. Chem. 256, 8039-8044.
- COX, M. M. AND LEHMAN, I. R. (1981) Proc. Natl. Acad. Sci. USA 78, 3433-3437.
- SOLTIS, D. A. AND LEHMAN, I. R. (1983) J. Biol. Chem. 258, 6073-6077.
- RADDING, D. M. (1991) J. Biol. Chem. 266, 5355 - 5358.
- DI CAPUA, E., ENGEL, A., STASIAK, A. AND KOLLER, T. (1982) J. Mol. Biol. 157, 87-103.
- REGISTER, J. C. AND GRIFFITH, J. (1986) Proc. Natl. Acad. Sci. USA 83, 624-628.
- SANCAR, A., STACHELEK C., KONIGSBERG, W. AND RUPP, W. D. (1980) Proc. Natl. Acad. Sci. USA 77, 2611-2615.
- WASSERMAN, S. A. AND COZZARELLI, N. R. (1985) Proc. Natl. Acad. Sci. USA 82, 1079-1083.
- SHORTLE, D., KOSHLAND, D., WEINSTOCK, G. M. AND BOTSTEIN, D. (1980) Proc. Natl. Acad. Sci. USA 77, 5375-5379.
- HONIGBERG, S. M., RAO, B. J. AND RADDING, C. M. (1986) Proc. Natl. Acad. Sci. USA 83, 9586-9590.
- RIGAS, B., WELCHER, A. A., WARD, D. C. AND WEISSMAN, S. M. (1986) Proc. Natl. Acad. Sci. USA 83, 9591-9595.
Applications:
- Analysis of complex DNA structures by electron microscopy(9)
- Site-directed mutagenesis(10)
- Screening of DNA libraries(11,12)
Storage:
Shipped on dry ice. For long term storage store at -70°C. Avoid repeated freeze/thaw cycles.