Genetic Performance Certified
Agarose - Low Melt, Separation ≤ 1000 bp
Genetic Performance Certified
MB Grade
PRODUCT SPECIFICATIONS
Form: Granular, free-flowing powder
EEO (-mr): ≤ 0.10
Gel Point (4%): ≤ 35°C
Remelt Point (4%): ≤ 65°C
Gel Strength (4%): ≥ 1000 gm/cm2
Moisture: ≤ 5%
Residue on Ignition: ≤ 0.3%
Sulfate (SO4): ≤ 0.12%
Genetic Performance Tests:
DNase: Passes test
RNase: Passes test
In-Gel Restriction Enzyme Digestion: Passes test
In-Gel Ligation: Passes test
DNA Binding: Passes test
Gel Background: Passes test
DNA Resolution: Passes test
Agarase Digestion: Passes test
Separation Guidelines:
Range of separation in 1X TAE:
2.0%: 1500 - 100 bp
3.0%: 700 - 150 bp
4.0%: 300 - 70 bp
5.0%: 50 - 10 bp
Range of separation in 1X TBE:
2.0%: 1000 - 400 bp
3.0%: 500 - 100 bp
4.0%: 150 - 10 bp
5.0%: < 30 bp
Applications:
- Enzymatic modification of nucleic acids in re-melted gels.
- Separation of DNA or RNA fragments ≤ 1000 bp.
- In-gel recovery of nucleic acid fragments.
- PCR analysis.
- Labile sample procedures.
Isolating DNA from low-melting temperature agarose:
Follow procedure for preparing an agarose gel and be sure to run the electrophoresis at 4°C to keep the gel from melting. Excise the DNA band of interest and transfer it to a microcentrifuge tube. Cut as close to the DNA band as possible to avoid any unnecessary agarose. Add 5 volumes of 20mM Tris-HCl (pH 8.0), 1mM EDTA (pH 8.0) to the tube with the agarose. Cap the tube and heat for 5 minutes at 65°C. The agarose should melt. Cool the solution to room temperature and add an equal volume of equilibrated phenol. Vortex for 20 seconds and spin at 400 x g for 10 minutes (20°C). Remove the aqueous phase (top) to a new microcentrifuge tube. Re-extract with phenol:chloroform and then with chloroform. Precipitate the final aqueous phase with 0.2 volumes of 10mM ammonium acetate and 2 volumes of ethanol. Recover the DNA by centrifugation.
CAS:
9012-36-6
MSDS / Material Safety Data Sheets
Agarose - Low Melt, Separation < 1000 bp Genetic Performance Certified_MSDS
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