RNase A, Solution
(E.C.3.1.27.5)
Source:
Bovine pancreas
Description:
RNase A specifically cleaves single-stranded RNA at 3' phosphate linkages of pyrimidine residues leaving pyrimidine 3' phosphates and oligonucleotides with terminal pyrimidine 3' phosphates(1).
Properties:
Molecular Weight: 13.7 kDa
Optimum pH: 7.0-7.5
Inhibitors: Heavy metal ions, RNase Inhibitors, vanadyl-ribonucleoside complexes.
Purity:
The enzyme is chromatographically purified. Aggregate free, monophoretic on native gel electrophoresis.
Storage Buffer:
0.1M phosphate buffer containing 0.1% v/v phenol as a preservative.
Unit Definition:
One unit hydrolyzes yeast RNA liberating soluble oligonucleotide causing an increase in absorbance at260 nm of 1.0 at 37°C, pH 5.0.
Concentration:
10-20 mg/ml
References:
- DAVIDSON, J. N. (1972) The Biochemistry of the Nucleic Acids, 7th edition, (Academic Press, New York).
- MYERS, R. M., LARIN, Z. AND MANIATIS, T. (1985) Science, 230, 1242-1246.
Applications:
- Cleaving unhybridized areas of RNA from RNA:DNA hybrids in RNA or DNA
mapping(2). - Removing contaminating RNA from DNA mini-preps. (Add 1 μl of a 10 mg/ml
RNase A solution to resuspended DNA pellet prepared from a 1.5 ml bacterial culture.)
Storage:
Shipped on dry ice. Store at -20°C.