PrepEase Plasmid 96-well Plate Kit
Designed for the isolation of high copy plasmid DNA using 96 well plates
Binding Capacity up to 20 μg
Culture Volume up to 5 ml
Designed for the isolation of high copy plasmid DNA using 96 well plates
- Based on familiar silica-membrane technology
- Time-saving purification of multiple samples using 96-well plates
- For use with plasmids up to 15 kb
- Yields up to 20 μg highly pure plasmid DNA from 5 ml of culture; 5 – 15 μg from 1.5 ml of culture
- Includes square-well blocks and cover sheets for bacterial cultivation, filter plates, binding plates, wash plates and buffers
The PrepEase® Plasmid 96-well Plate Kit enables rapid, simple purification of plasmid DNA from small volumes of bacterial cultures. The kit uses an alkaline lysis procedure, followed by vacuum filtration (and optionally, centrifugation) with filter and plasmid binding plates. The plasmid binding plates contain patented anion-exchange membrane filters, allowing highly specific purification of plasmid DNA.
The PrepEase Plasmid 96-well Plate Kit requires a vacuum manifold, such as the PrepEase® Vacuum Manifold or the QiaVac™ 96. When using the QiaVac 96 manifold, a PrepEase® Vacuum Plate Frame is required to adjust the height of the wash and elution plates.
Plasmids ranging up to 15 kb in size may be purified with yields of up to 15 μg from 1.5 ml of bacterial culture. The kit is suited for high copy number plasmid purification. Low copy plasmids may be purified by increasing the culture volumes to 5.0 ml. Simple modifications of the protocol enable use of a wide variety of bacterial strains and growth media, elimination of problematic nucleases and optimization of washes to increase yield.
Properties:
Ships ambient. Store at room temperature.
MSDS / Material Safety Data Sheets
PrepEase Plasmid 96-well Plate Kit_MSDS
Protocol, Brief
PrepEase Plasmid 96-well Plate Kit_Brief Protocol
Protocol, Long
PrepEase Plasmid 96-well Plate Kit_Protocol