CHAPS, Anagrade
3-[(3-Cholamidopropyl)-Dimethylammonio]-1-Propane Sulfonate/N,N-Dimethyl-3-Sulfo-N-[3-[[3α,5β,7α,12α)-3,7,12-Trihydroxy-24-Oxocholan-24-yl]Amino]propyl]-1-Propanaminium Hydroxide, Inner Salt
C316: Nondenaturing zwitterionic detergent for functional solubilization and purification of membrane protein
Molecular Formula:
C32H58N2O7S
Molecular Weight:
614.9
CMC (H2O): ~ 8 mM(1) (0.49%)
Aggregation Number (H2O)(2): ~ 10
dn/dc (H2O)(3): 0.1323 mL/gM
Purity: ≥ 99% by HPLC analysis.
pH (1% solution): 5-8
Solubility in water: ≥ 0.5M (30.7%)
Conductance (0.5 M solution): < 50 μS
Percent fluorescence due to a 0.1% detergent solution at 345 nm: < 10
Absorbance of a 1% detergent solution:
340 nm: < 0.02
280 nm: < 0.04
260 nm: < 0.06
1. Hjelmeland, L. M., Nebert, D. W. and Osborne, Jr., J. C. (1983) Anal. Biochem. 130, 72-82.
2. Womack, M. D., Kendall, D. A. and MacDonald, R. C. (1983) Biochim. Biophys. Acta 733, 210-215.
3. Measurement obtained in collaboration with Professor Mark Foster (University of Akron) under an experimental services contract
4. Bellis, S. L., Kass-Simon, G. and Rhoads, D. E. (1992) Biochem. 31, 9838-9843.
CAS:
75621-03-3
